Question 1

The conserved adenine nucleotide in introns serves as the:

Accepted Answer

A)  site for intron recognition for the spliceosome.
B)  branch point for the formation of the intronic "lariat."
C)  key point for ribosome assembly and initiation of translation.
D)  initial site of intronic RNA digestion and removal.
E)  molecular signal for RNA splicing.F) C) and D)
G) A) and B)

Question 2

Following splicing,the conserved adenosine nucleotide within the intron displays an unusual array of bonds,including:

Accepted Answer

A)  a phosphate attached to the 4-carbon of the adenine nitrogenous base.
B)  phosphodiester bonds attached at three places on the ribose (2′,3′,5′) .
C)  additional methyl (-CH3) groups added to the adenine.
D)  a DNA strand attached to the spliced RNA.
E)  attachment to an arginine amino acid.F) C) and D)
G) B) and C)

Question 3

Template strand DNA and encoded RNA are:

Accepted Answer

A)  complimentary of one another with antiparallel orientation.
B)  complimentary of one another but share the same 5′-3′ orientation.
C)  identical sequences with the exception of U substituted for T.
D)  different with regard to the inclusion of introns.
E)  interconverted via nucleic acid remodeling.F) B) and C)
G) C) and D)

Question 4

In a chromosome,which of the following is TRUE?

Accepted Answer

A)  RNAs of different genes can be transcribed off either DNA strand,but always 5' to 3'.
B)  RNAs of different genes can be transcribed off either DNA strand,but always 3' to 5'.
C)  The RNAs of all genes are synthesized 5' to 3' off the same DNA strand.
D)  The RNAs of all genes are synthesized 3' to 5' off the same DNA strand.
E)  Different genes can be transcribed off either strand,some in the 5' to 3' direction and some in the 3' to 5' direction.F) A) and B)
G) B) and D)

Question 5

Suppose that you have sequenced five genes,including the putative promoter region of each gene,that are involved in the cold acclimation process in Arabidopsis.Each of the five genes is expressed only when the temperature falls below a certain temperature.You know that a specific transcription factor (CBF1)binds somewhere between -200 and -250 of these genes to initiate transcription.How could you use the sequences to determine the DNA sequence motif that is bound by CBF1?

Accepted Answer

Align the five sequences and look for a ...

Question 6

Which of the following is/are role(s) of the 5′ cap?

Accepted Answer

A)  The cap helps the RNA polymerase find the promoter and initiate transcription.
B)  The cap plays a role in the removal of introns.
C)  The cap acts as a binding site for the ribosome.
D)  The cap protects the RNA from degradation.
E)  The cap acts as a binding site for the ribosome and protects the RNA from degradation.F) A) and C)
G) D) and E)

Question 7

The functional equivalent of the TATA binding protein in prokaryotes is:

Accepted Answer

A)  a sigma subunit.
B)  a holoenzyme.
C)  the Rho factor.
D)  TFIID.
E)  hairpin loops.F) A) and B)
G) A) and C)

Question 8

Of the three classes of functional RNAs,rRNA and tRNA exist in both eukaryotes and prokaryotes.However,snRNAs exist only in one of these types of organisms.Do snRNAs exist in prokaryotes or eukaryotes? Explain why they are only found in that type of organism.

Accepted Answer

Eukaryotes.snRNAs play a role ...

Question 9

The -10 and -35 boxes found in most bacterial promoter elements were discovered by:

Accepted Answer

A)  comparing the DNA sequences in front of highly expressed genes and determining nucleic acids that were common (consensus) .
B)  the finding that RNA polymerase begins translation when a 10-nucleotide followed by a 35-nucleotide repeat of guanine ("G") are encountered.
C)  comparing bacterial promoters to known eukaryotic promoters.
D)  examining data from the human genome project.
E)  Robert Koch as he characterized the microbe that causes tuberculosis.F) B) and D)
G) C) and D)

Question 10

Which of the following is/are TRUE about functional RNA?

Accepted Answer

A)  Functional RNAs do not encode proteins and are active as RNA.
B)  Some functional RNAs encode proteins.
C)  Examples of functional RNA include mRNA,tRNA,and rRNA.
D)  Examples of functional RNA include tRNA,rRNA,snRNA,and miRNA.
E)  Functional RNAs do not encode proteins,and examples include tRNA,rRNA,snRNA,and miRNA.F) B) and D)
G) D) and E)

Question 11

The role of most microRNAs within a eukaryotic cell is to:

Accepted Answer

A)  regulate the splicing of primary transcripts to mRNAs.
B)  bind with other RNAs to stabilize their secondary structure.
C)  associate with ribosomal proteins to facilitate translation.
D)  regulate RNA polymerase activity in the nucleus.
E)  repress the expression of genes by destroying mRNAs.F) B) and E)
G) A) and B)

Question 12

Pulse-chase experiments provided evidence that:

Accepted Answer

A)  RNA is made of ribose sugars rather than deoxyribose.
B)  RNA is an information-transfer intermediary between DNA and protein.
C)  many viruses have RNA genomes.
D)  RNA is capable of catalyzing biological reactions.
E)  RNA is synthesized in short pulses of transcriptional activity.F) None of the above
G) B) and C)

Question 13

What would the minimum word (codon)size be if the number of different bases in the mRNA were a)two;b)three;c)five.Give your reasoning.Assume that all other features of the translation of mRNA into protein are the same as what we now know.

Accepted Answer

Note: The number of ...

Exam 8: Rna: Transcription and Processing

In a chromosome,which of the following is TRUE?

Correct Answer
verified

The conserved adenine nucleotide in introns serves as the:

Correct Answer
verified

What would the minimum word (codon)size be if the number of different bases in the mRNA were a)two;b)three;c)five.Give your reasoning.Assume that all other features of the translation of mRNA into protein are the same as what we now know.

Correct Answer
verified
Note: The number of ...
View Answer

Of the three classes of functional RNAs,rRNA and tRNA exist in both eukaryotes and prokaryotes.However,snRNAs exist only in one of these types of organisms.Do snRNAs exist in prokaryotes or eukaryotes? Explain why they are only found in that type of organism.

Correct Answer
verified
Eukaryotes.snRNAs play a role ...
View Answer

Correct Answer
verified
Align the five sequences and look for a ...
View Answer

Which of the following is/are TRUE about functional RNA?

Correct Answer
verified

The functional equivalent of the TATA binding protein in prokaryotes is:

Correct Answer
verified

Which of the following is/are role(s) of the 5′ cap?

Correct Answer
verified

Following splicing,the conserved adenosine nucleotide within the intron displays an unusual array of bonds,including:

Correct Answer
verified

The -10 and -35 boxes found in most bacterial promoter elements were discovered by:

Correct Answer
verified

The role of most microRNAs within a eukaryotic cell is to:

Correct Answer
verified

Pulse-chase experiments provided evidence that:

Correct Answer
verified

Template strand DNA and encoded RNA are:

Correct Answer
verified

Exam 8: Rna: Transcription and Processing

In a chromosome,which of the following is TRUE?

Correct AnswerverifiedShow Answer

The conserved adenine nucleotide in introns serves as the:

Correct AnswerverifiedShow Answer

What would the minimum word (codon)size be if the number of different bases in the mRNA were a)two;b)three;c)five.Give your reasoning.Assume that all other features of the translation of mRNA into protein are the same as what we now know.

Correct AnswerverifiedNote: The number of ...View AnswerShow Answer

Of the three classes of functional RNAs,rRNA and tRNA exist in both eukaryotes and prokaryotes.However,snRNAs exist only in one of these types of organisms.Do snRNAs exist in prokaryotes or eukaryotes? Explain why they are only found in that type of organism.

Correct AnswerverifiedEukaryotes.snRNAs play a role ...View AnswerShow Answer

Correct AnswerverifiedAlign the five sequences and look for a ...View AnswerShow Answer

Which of the following is/are TRUE about functional RNA?

Correct AnswerverifiedShow Answer

The functional equivalent of the TATA binding protein in prokaryotes is:

Correct AnswerverifiedShow Answer

Which of the following is/are role(s) of the 5′ cap?

Correct AnswerverifiedShow Answer

Following splicing,the conserved adenosine nucleotide within the intron displays an unusual array of bonds,including:

Correct AnswerverifiedShow Answer

The -10 and -35 boxes found in most bacterial promoter elements were discovered by:

Correct AnswerverifiedShow Answer

The role of most microRNAs within a eukaryotic cell is to:

Correct AnswerverifiedShow Answer

Pulse-chase experiments provided evidence that:

Correct AnswerverifiedShow Answer

Template strand DNA and encoded RNA are:

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Note: The number of ...
View Answer

Correct Answer
verified
Eukaryotes.snRNAs play a role ...
View Answer

Correct Answer
verified
Align the five sequences and look for a ...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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