Question 1

Two different nucleic acids can _____ by uniting at their complementary sites.

Accepted Answer

The answer of Two different nucleic acids can _____ by...

Question 2

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Accepted Answer

A)  Escherichia coli
B)  Saccharomyces cerevisiae
C)  Thermus aquaticus
D)  Pseudomonas syringae
E)  Pseudomonas fluorescensF) A) and B)
G) None of the above

Question 3

Which step involves transformation?

Accepted Answer

A)  target DNA removed from cells and isolated
B)  cloning host takes up a plasmid
C)  target DNA and plasmid treated with the same restriction endonuclease
D)  desired protein is produced by cloning host
E)  gene is amplified by multiplication of cloning hostF) A) and E)
G) A) and D)

Question 4

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Accepted Answer

The answer of The _____ blot method analyzes DNA, while...

Question 5

Amplification of DNA is accomplished by

Accepted Answer

A)  polymerase chain reaction.
B)  DNA sequencing.
C)  gene probes.
D)  Southern blot.
E)  All of the choices are correct.F) A) and B)
G) A) and C)

Question 6

Compare and contrast recombinant DNA and gene therapy.

Accepted Answer

Recombinant DNA and gene therapy are bot...

Question 7

EcoRI and HindIII are

Accepted Answer

A)  palindromes.
B)  reverse transcriptase.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) None of the above
G) All of the above

Question 8

The function of the dideoxy (dd)  nucleotides that are used in the Sanger method of DNA sequencing is to

Accepted Answer

A)  denature DNA into single strands.
B)  serve as primers.
C)  be a fluorescent tag.
D)  incorporate into newly replicated DNA strands and stop elongation.
E)  None of the choices are correct.F) A) and B)
G) B) and E)

Question 9

Which PCR step synthesizes complementary DNA strands?

Accepted Answer

A)  add DNA polymerase and nucleotides at 72°C
B)  cool DNA to between 50°C and 65°C
C)  add primers
D)  heat target DNA to 94°C
E)  repeat the cycle of heating and coolingF) A) and B)
G) C) and D)

Question 10

In order to ensure compatibility between the target DNA and the plasmid DNA,

Accepted Answer

A)  target DNA is removed from cells and isolated.
B)  cloning host is treated with calcium chloride and receives plasmid.
C)  target DNA and plasmid are treated with the same restriction endonuclease.
D)  desired protein is produced by cloning host.
E)  gene is amplified by multiplication of cloning host.F) D) and E)
G) C) and D)

Question 11

Geneticists can make complimentary DNA copies of messenger, transfer, and ribosomal RNA by using

Accepted Answer

A)  palindromes.
B)  reverse transcriptase.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) A) and C)
G) D) and E)

Question 12

Analysis of DNA fragments in gel electrophoresis involves

Accepted Answer

A)  larger fragments move slowly and remain closer to the wells.
B)  DNA has an overall negative charge and moves to the positive pole.
C)  DNA fragments are stained to see them.
D)  an electric current through the gel causes DNA fragments to migrate.
E)  All of the choices are correct.F) D) and E)
G) A) and D)

Question 13

The enzyme___ adds three nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated

Accepted Answer

A)  DNA polymerase III, 3'->5'
B)  DNA polymerase I, 3'->5'
C)  DNA polymerase III, 5'->3'
D)  DNA polymerase I, 5'->3'E) B) and C)
F) A) and D)

Question 14

If you have the sequence of mRNA that is AUAUGC, then the DNA antisense strand is ____.

Accepted Answer

A)  AUAUGC
B)  UAUACG
C)  TATACG
D)  CGUAUAE) B) and C)
F) None of the above

Question 15

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Accepted Answer

A DNA fingerprint is a unique pattern of...

Question 16

This is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci:

Accepted Answer

A)  cloning
B)  gene therapy
C)  antisense therapeutic
D)  DNA fingerprinting
E)  None of the choices are correct.F) C) and E)
G) D) and E)

Question 17

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 18

The polymerase chain reaction (PCR)  has been used in all of the following fields, except

Accepted Answer

A)  forensics.
B)  evolutionary studies.
C)  gene mapping.
D)  medicine.
E)  None of the choices; it is useful for all these fields.F) B) and D)
G) A) and B)

Question 19

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from
E.coli cells growing in a lab culture.

Accepted Answer

1. Start by growing E. coli cells in a l...

Question 20

All of the following are desirable features in a microbial cloning host, except it

Accepted Answer

A)  can be grown in large quantities.
B)  has a slow growth rate.
C)  is capable of accepting plasmid vectors.
D)  will secrete a high yield of proteins from expressed foreign genes.
E)  has a genome that is well delineated.F) A) and D)
G) C) and D)

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Amplification of DNA is accomplished by

Correct AnswerverifiedShow Answer

The polymerase chain reaction (PCR) has been used in all of the following fields, except

Correct AnswerverifiedShow Answer

All of the following are desirable features in a microbial cloning host, except it

Correct AnswerverifiedShow Answer

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Correct AnswerverifiedShow Answer

If you have the sequence of mRNA that is AUAUGC, then the DNA antisense strand is ____.

Correct AnswerverifiedShow Answer

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Correct AnswerverifiedShow Answer

The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to

Correct AnswerverifiedShow Answer

In order to ensure compatibility between the target DNA and the plasmid DNA,

Correct AnswerverifiedShow Answer

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

Correct AnswerAnswered by ExamLex AI1. Start by growing E. coli cells in a l...View AnswerShow Answer

This is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci:

Correct AnswerverifiedShow Answer

When DNA is heated, the two strands will separate.

Correct AnswerverifiedShow Answer

Geneticists can make complimentary DNA copies of messenger, transfer, and ribosomal RNA by using

Correct AnswerverifiedShow Answer

EcoRI and HindIII are

Correct AnswerverifiedShow Answer

Analysis of DNA fragments in gel electrophoresis involves

Correct AnswerverifiedShow Answer

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Correct AnswerAnswered by ExamLex AIA DNA fingerprint is a unique pattern of...View AnswerShow Answer

Compare and contrast recombinant DNA and gene therapy.

Correct AnswerAnswered by ExamLex AIRecombinant DNA and gene therapy are bot...View AnswerShow Answer

Two different nucleic acids can _____ by uniting at their complementary sites.

Correct AnswerverifiedShow Answer

The enzyme___ adds three nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complementary DNA strands?

Correct AnswerverifiedShow Answer

Which step involves transformation?

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The _ blot method analyzes DNA, while the _ method analyzes RNA.

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Correct Answer
Answered by ExamLex AI
1. Start by growing E. coli cells in a l...
View Answer

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Correct Answer
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Correct Answer
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Correct Answer
Answered by ExamLex AI
A DNA fingerprint is a unique pattern of...
View Answer

Correct Answer
Answered by ExamLex AI
Recombinant DNA and gene therapy are bot...
View Answer

Correct Answer
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The enzyme_ adds three nucleotides of DNA in the _direction when DNA is being semi-conservatively replicated

Correct Answer
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Correct Answer
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Correct Answer
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