Question 1

Bacteria that require special growth factors and complex nutrients are termed ______.

Accepted Answer

A)   fermentative
B)   hemolytic
C)   fastidious
D)   aerobic
E)   anaerobicF) A) and C)
G) A) and B)

Question 2

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following components would likely be present in a selective medium for this purpose?

Accepted Answer

A)   Thioglycolic acid
B)   Sheep red blood cells
C)   Bile salts
D)   Peptone
E)   NaClF) A) and E)
G) C) and D)

Question 3

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Accepted Answer

A)   0.2 mm
B)   0.1 µm
C)   2.0 µm
D)   0.2 µmE) All of the above
F) B) and C)

Question 4

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

A)   ocular; oil immersion
B)   scanning; objective
C)   ocular; objective
D)   objective; ocularE) B) and D)
F) None of the above

Question 5

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A)   Transmission electron
B)   Differential interference contrast
C)   Scanning electron
D)   Phase-contrast
E)   FluorescenceF) A) and B)
G) C) and D)

Question 6

Eosin-methylene blue agar,or EMB,is a commonly used bacteriological medium for growing gram-negative bacteria from complex environments.It contains two dyes,eosin and methylene blue,as well as the sugar lactose.Eosin is a pH indicator that changes color when the medium is acidic,while methylene blue inhibits the growth of most gram-positive bacteria.If an organism consumes lactose,acid will be produced,causing the bacterial colonies to change color. Based upon this description,this medium is ______.

Accepted Answer

A)   reducing
B)   for enrichment
C)   selective
D)   differential and selective
E)   differentialF) A) and C)
G) All of the above

Question 7

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

A) True 
 B)False

Question 8

If a microbiologist is studying a specimen at a total magnification of 950X,what is the magnifying power of the objective lens if the ocular lens is 10X?

Accepted Answer

A)   95X
B)   940X
C)   950X
D)   9500XE) A) and D)
F) None of the above

Question 9

Which of the following is not a benefit of agar as a solid medium?

Accepted Answer

A)   Holds moisture
B)   Flexibility
C)   Solid at room temperature
D)   Can be inoculated and poured at a temperature that is not harmful
E)   Easily digested by most microbesF) A) and C)
G) All of the above

Question 10

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)   Enriched
B)   Enumeration
C)   Selective
D)   Reducing
E)   DifferentialF) None of the above
G) B) and C)

Question 11

Mannitol salt agar is selective for organisms in which bacterial genus?

Accepted Answer

A)   Salmonella
B)   Staphylococcus
C)   Escherichia
D)   Neisseria
E)   StreptococcusF) B) and D)
G) A) and B)

Question 12

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

Accepted Answer

A)   920  μ\muμ m
B)   0.2  μ\muμ m
C)  175  μ\muμ m
D)   57.5  μ\muμ m
E)   4,000  μ\muμ m F) A) and E)
G) A) and D)

Question 13

A microbiology student with a visual disability is viewing a sample of Bacillus endospores in a phase contrast microscope that is linked to an iPad.By "stretching" the image on the iPad screen,the student can increase the apparent size of the endospores.How does this manipulation affect resolution and contrast? (No software is being used to alter the image!)

Accepted Answer

A)   Only the magnification can be increased; resolution and contrast are unchanged.
B)   Contrast will improve because the microscope is phase-contrast.
C)   Both magnification and resolution increase; contrast is unchanged.
D)   Resolution and magnification will decrease because the screen is not as good as the human eye.E) A) and B)
F) A) and C)

Question 14

Which type of microscope cannot image live specimens?

Accepted Answer

A)   Differential interference
B)   Bright-field
C)   Electron
D)   Dark-field
E)   Phase-contrastF) None of the above
G) B) and C)

Question 15

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Accepted Answer

A)   Contrast
B)   Magnification
C)   Refraction
D)   ResolutionE) A) and B)
F) A) and C)

Question 16

The purpose of staining cells on a microscope slide is to ______.

Accepted Answer

A)   secure them to the slide
B)   kill them
C)   observe motility
D)   enlarge the cells
E)   add contrast to see them betterF) A) and B)
G) A) and E)

Question 17

The Five I's of studying microorganisms include all of the following except ______.

Accepted Answer

A)   identification
B)   isolation
C)   incubation
D)   infection
E)   inoculationF) A) and B)
G) B) and E)

Question 18

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Accepted Answer

A)   inoculation
B)   contamination
C)   infection
D)   immunization
E)   isolationF) A) and C)
G) D) and E)

Question 19

All of the following pertain to the fluorescence microscope except

Accepted Answer

A)   it is a type of compound microscope.
B)   it is used to diagnose certain infections.
C)   it uses electrons to produce a specimen image.
D)   it requires the use of dyes like acridine or fluorescein.
E)   it requires an ultraviolet radiation source.F) A) and E)
G) A) and D)

Question 20

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

The Five I's of studying microorganisms include all of the following except ______.

Correct Answer
verified

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following components would likely be present in a selective medium for this purpose?

Correct Answer
verified

If a microbiologist is studying a specimen at a total magnification of 950X,what is the magnifying power of the objective lens if the ocular lens is 10X?

Correct Answer
verified

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Correct Answer
verified

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Correct Answer
verified

The purpose of staining cells on a microscope slide is to ______.

Correct Answer
verified

Correct Answer
verified

Which of the following is not a benefit of agar as a solid medium?

Correct Answer
verified

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

Correct Answer
verified

Which type of microscope cannot image live specimens?

Correct Answer
verified

All of the following pertain to the fluorescence microscope except

Correct Answer
verified

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Correct Answer
verified

Mannitol salt agar is selective for organisms in which bacterial genus?

Correct Answer
verified

The procedures for culturing a microorganism require the use of a microscope.

Correct Answer
verified

Mixed cultures are also referred to as contaminated cultures.

Correct Answer
verified

Bacteria that require special growth factors and complex nutrients are termed ______.

Correct Answer
verified

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct Answer
verified

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Correct Answer
verified

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Correct Answer
verified

Correct Answer
verified

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

The Five I's of studying microorganisms include all of the following except ______.

Correct AnswerverifiedShow Answer

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following components would likely be present in a selective medium for this purpose?

Correct AnswerverifiedShow Answer

If a microbiologist is studying a specimen at a total magnification of 950X,what is the magnifying power of the objective lens if the ocular lens is 10X?

Correct AnswerverifiedShow Answer

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Correct AnswerverifiedShow Answer

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Correct AnswerverifiedShow Answer

The purpose of staining cells on a microscope slide is to ______.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which of the following is not a benefit of agar as a solid medium?

Correct AnswerverifiedShow Answer

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

Correct AnswerverifiedShow Answer

Which type of microscope cannot image live specimens?

Correct AnswerverifiedShow Answer

All of the following pertain to the fluorescence microscope except

Correct AnswerverifiedShow Answer

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Correct AnswerverifiedShow Answer

Mannitol salt agar is selective for organisms in which bacterial genus?

Correct AnswerverifiedShow Answer

The procedures for culturing a microorganism require the use of a microscope.

Correct AnswerverifiedShow Answer

Mixed cultures are also referred to as contaminated cultures.

Correct AnswerverifiedShow Answer

Bacteria that require special growth factors and complex nutrients are termed ______.

Correct AnswerverifiedShow Answer

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct AnswerverifiedShow Answer

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Correct AnswerverifiedShow Answer

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified