Question 1

In choosing the vector used to carry human genes into a host cell,which of the following should be the important consideration?

Accepted Answer

A)   How much donor DNA can be carried on the vector
B)   What kind of plasmid it is
C)   Whether the vector comes from a bacterium or a virus
D)   Whether the vector contains RNA or DNAE) A) and B)
F) A) and C)

Question 2

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 3

Micro RNA therapy is based on the premise that

Accepted Answer

A)  	miRNA molecules function to regulate gene expression, usually by inhibiting transcription; a non-functional miRNA can be replaced thereby reducing unwanted protein production.
B)  	miRNA molecules function to regulate gene expression, usually by promoting translation; a non-functional miRNA can be repaired thereby reducing unwanted protein production.
C)  	miRNA molecules function to regulate gene expression, usually by initiating transcription; an over-production of miRNA can be replaced thereby promoting necessary protein production.
D)  	miRNA molecules function to regulate protein production, usually by inhibiting translation; a hyperactive miRNA can be replaced thereby reducing unwanted proteins.E) C) and D)
F) B) and D)

Question 4

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA fingerprinting techniques.The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

Accepted Answer

A)   miRNAs
B)   frameshift mutations
C)   SNPs
D)   microarraysE) A) and C)
F) All of the above

Question 5

Transgenic organisms are ______.

Accepted Answer

A)   created in nature
B)   only microorganisms
C)   copyrighted
D)   patentedE) A) and D)
F) B) and C)

Question 6

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Accepted Answer

A)   principal sources of restriction endonucleases.
B)   used as cloning vectors.
C)   genetically engineered bacteria.
D)   sources of heat-stable DNA polymerases.E) A) and B)
F) C) and D)

Question 7

When DNA is heated,the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 8

If you start with 3 double-stranded DNA fragments,after 4 cycles of PCR you will have ____ fragments.

Accepted Answer

A)   12
B)   24
C)   27
D)   48
E)   81F) C) and D)
G) C) and E)

Question 9

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Accepted Answer

A)   DNA
B)   libraries
C)   clones
D)   digests
E)   booksF) A) and E)
G) All of the above

Question 10

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Accepted Answer

A) True 
 B)False

Question 11

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)  Add DNA polymerase and nucleotides at 72° C.
B)  Cool DNA to between 50° C and 65° C.
C)  Add primers.
D)  Heat target DNA to 94° C.
E)  Repeat the cycle of heating and cooling.F) None of the above
G) A) and B)

Question 12

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 13

Transgenic animals

Accepted Answer

A)   can be engineered to become factories for manufacturing proteins.
B)   are often obtained from germline engineering.
C)   will pass the genes on to their offspring.
D)   commonly include mice.
E)   All of the choices are correct.F) All of the above
G) A) and C)

Question 14

In the formation of recombinant DNA,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Accepted Answer

A)   DNA polymerase I
B)   DNA polymerase II
C)   DNA helicase
D)   DNA ligase
E)   PrimaseF) A) and B)
G) A) and E)

Question 15

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) A) and D)
G) B) and E)

Question 16

When micro RNA molecules malfunction,their effect is limited to the inhibition of protein production,which results in tumor growth.

Accepted Answer

A) True 
 B)False

Question 17

Your infant has been diagnosed with severe combined immunodeficiency disease (SCID) .Your doctor suggests which treatment as a possible cure for the disease?

Accepted Answer

A)   Genome mapping
B)   Reverse transcription
C)   Gene therapy
D)   MicroarraysE) A) and C)
F) A) and D)

Question 18

Which of the following recombinant DNA technology products is mismatched?

Accepted Answer

A)   Erythropoietin - stimulates erythrocyte production in bone marrow
B)   Factor VIII - a protein necessary for the coagulation cascade
C)   Interferons - stimulate antiviral protein production
D)   Tissue plasminogen activating factor - stimulates blood clottingE) None of the above
F) B) and C)

Question 19

Which step in gene mapping can occur even more rapidly by PCR?

Accepted Answer

A)   Target DNA removed from cells and isolated
B)   Cloning host treated with calcium chloride and receives plasmid
C)   Target DNA and plasmid treated with the same restriction endonuclease
D)   Desired protein is produced by cloning host
E)   Gene is amplified by multiplication of cloning hostF) None of the above
G) B) and D)

Question 20

A proteome is an organism's full array of expressed proteins,and proteomics is the study of those proteins and the functions they mediate.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering and Recombinant Dna

Genomic _____ are collections of isolated genes maintained in cloning hosts.

Correct AnswerverifiedShow Answer

Your infant has been diagnosed with severe combined immunodeficiency disease (SCID) .Your doctor suggests which treatment as a possible cure for the disease?

Correct AnswerverifiedShow Answer

In the formation of recombinant DNA,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Correct AnswerverifiedShow Answer

If you start with 3 double-stranded DNA fragments,after 4 cycles of PCR you will have ____ fragments.

Correct AnswerverifiedShow Answer

Transgenic organisms are ______.

Correct AnswerverifiedShow Answer

Which of the following recombinant DNA technology products is mismatched?

Correct AnswerverifiedShow Answer

In choosing the vector used to carry human genes into a host cell,which of the following should be the important consideration?

Correct AnswerverifiedShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA fingerprinting techniques.The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

When micro RNA molecules malfunction,their effect is limited to the inhibition of protein production,which results in tumor growth.

Correct AnswerverifiedShow Answer

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Correct AnswerverifiedShow Answer

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Correct AnswerverifiedShow Answer

Which step in gene mapping can occur even more rapidly by PCR?

Correct AnswerverifiedShow Answer

Transgenic animals

Correct AnswerverifiedShow Answer

Micro RNA therapy is based on the premise that

Correct AnswerverifiedShow Answer

After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

A proteome is an organism's full array of expressed proteins,and proteomics is the study of those proteins and the functions they mediate.

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Correct AnswerverifiedShow Answer

When DNA is heated,the two strands will separate.

Correct AnswerverifiedShow Answer

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