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EcoRI is a restriction endonuclease that recognizes the hexanucleotide sequence, GAATTC. Identify the fragments cleaved by EcoRI in the given DNA strand. ? 5'---g-a-a-t-t-c-a-t-t-a---3' ⟶ ECORI \overset{\text { ECORI }}{\longrightarrow} ?


A)
5'---G + 5'-A-A-T-T-C-A-T-T-A---3'
B)
5'---G-A+5'-A-T-T-C-A-T-T-A---3'
C)
5'---G-A-A+5'-T-T-C-A-T-T-A---3'
D)
5'---G-A-A-A-T+5'-T-C-A-T-T-A---3'

E) A) and D)
F) B) and C)

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The following structures is a pyrimidine. The following structures is a pyrimidine.

A) True
B) False

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Which of the following is D-ribose? Which of the following is D-ribose? A) 1 B) 2 C) 3 D) 4


A) 1
B) 2
C) 3
D) 4

E) B) and D)
F) All of the above

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Provide the structure of guanine, a heterocyclic base found in DNA.

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How many bases are present in each strand of a complete turn of B-DNA?


A) 3
B) 7
C) 10
D) 15

E) All of the above
F) None of the above

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Number each position in the ribose below according to the normal convention. Number each position in the ribose below according to the normal convention.

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If ranked for largest to smallest, the correct order of DNA, mRNA, and tRNA would be DNA > mRNA > tRNA.

A) True
B) False

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A double-stranded DNA molecule that contains 22%, on a mole basis, T must also contain ___% G.

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What is the approximate length of a complete turn of a double helix of DNA?


A) 340 pm
B) 800 pm
C) 2000 pm
D) 3400 pm

E) None of the above
F) B) and D)

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Which of the following is a definition of messenger RNA (mRNA) ?


A) RNA found in the ribosome, the site in cells where synthesis of proteins takes place
B) RNA which carries a specific amino acid to the site of protein synthesis
C) RNA which has coded genetic information
D) RNA which signals cell death

E) A) and B)
F) None of the above

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What sequence is fully complementary to the following sequence? 5'-AGTCAATTC-3'

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MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside.


A) Sanger dideoxy method
B) GAUCGUAAA
C) Watson-Crick
D) translation
E) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
F) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
G) transcription
H) Maxam-Gilbert method
I) AUGGCUGAG
J) replication
K) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
L) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
M) AGATCGCTC

N) G) and H)
O) A) and E)

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Provide the structure of adenosine, a nucleoside.

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Identify the given structure. ​ Identify the given structure. ​ A) Guanine-uracil B) Guanine-cytosine C) Thymine-uracil D) Thymine-adenine


A) Guanine-uracil
B) Guanine-cytosine
C) Thymine-uracil
D) Thymine-adenine

E) None of the above
F) A) and D)

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MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside.


A) Sanger dideoxy method
B) GAUCGUAAA
C) Watson-Crick
D) translation
E) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
F) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
G) transcription
H) Maxam-Gilbert method
I) AUGGCUGAG
J) replication
K) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
L) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
M) AGATCGCTC

N) C) and L)
O) G) and K)

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Which of the following is a pyrimidine base?


A) guanine
B) adenine
C) thymine
D) triethylamine

E) None of the above
F) A) and D)

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MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a RNA base sequence with guanine at the 3' end. _____ is a pyrimidine nucleoside.


A) Sanger dideoxy method
B) GAUCGUAAA
C) Watson-Crick
D) translation
E) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a RNA base sequence with guanine at the 3' end. _____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
F) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a RNA base sequence with guanine at the 3' end. _____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
G) transcription
H) Maxam-Gilbert method
I) AUGGCUGAG
J) replication
K) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a RNA base sequence with guanine at the 3' end. _____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
L) MATCH each of the following terms to a structure from the list below. There is only one correct answer for each question and structures may be used more than once. Place the letter of the structure in the blank to the left of the corresponding term. -_____ is a RNA base sequence with guanine at the 3' end. _____ is a pyrimidine nucleoside. A) Sanger dideoxy method B) GAUCGUAAA C) Watson-Crick D) translation E) F) G) transcription H) Maxam-Gilbert method I) AUGGCUGAG J) replication K) L) M) AGATCGCTC
M) AGATCGCTC

N) E) and F)
O) C) and H)

Correct Answer

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Relative to the secondary structure of DNA, there is no restriction on the sequence of bases along a polynucleotide chain. The exact sequence carries the genetic information.

A) True
B) False

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What tetrapeptide is coded by the following sequence of mRNA? 5'-AUG-GUA-AUG-UUG-3'


A) Met-Val-Met-Leu
B) Ala-Val- Leu-Met
C) Met-Val-Ala-Leu
D) Ala-Ala-Met-Leu

E) All of the above
F) B) and D)

Correct Answer

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The G-C base pair is the weaker than the A-T base pair as it has two hydrogen bonds whereas the A-T base pair has three.

A) True
B) False

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